Publications

Prabhu, N, M Prabakaran, HT Ho, S Velumani, J Qiang, M Goutama and J Kwang: Monoclonal antibodies against the fusion peptide of Hemagglutinin protect mice from lethal Influenza A H5N1 Infection. Journal of Virology (in press).

Karuppannan, AK, MH Jong, SH Lee, Y Zhu, M Selvaraj, J Lau, Q Jia and J Kwang: Attenuation of Porcine circovirus2 in SPF piglets by abrogation of ORF3 function. Virology (in press).

He, F, Q Du, YF Ho and J Kwang: Immunohistochemical detection of Influenza virus infection in formalin-fixed tissues with anti-H5 monoclonal antibody recognizing FFWTILKP. Journal of Virological Methods 155: 25-33 (2009).

He, F, Y Ho, L Yu and J Kwang: WSSV ie1 promoter is more efficient than CMV promoter for H5 Hemagglutinin expression in baculovirus as a chicken vaccine. BMC Microbiology 8: 238 (2008).

He, F and J Kwang: Identification and characterization of a new E3 ubiquitin ligase in white spot syndrome virus involved in virus latency. Virology Journal 5: 151 (2008).

Wei, L, LL Chee, T Wei, J Kwang, J Zhou, J Wang, L Shi and J Liu: The VP1 protein of avian encephalomyelitis virus is a major host-protective immunogen that serves as diagnostic potential. Journal of Virological Methods 149: 56-62 (2008a).

Wei, L, J Kwang, J Wang, L Shi, B Yang, Y Li and J Liu: Porcine circovirus type 2 induces the activation of nuclear factor kappa B by IκBα degradation. Virology 378: 177-184 (2008b).

Prabakaran, M, S Velumani, F He, AK Karuppannan, GY Geng, LK Yin and J Kwang: Protective immunity against influenza H5N1 virus challenge in mice by intranasal co-administration of baculovirus surface-displayed HA and recombinant CTB as an adjuvant. Virology 380: 412-420 (2008).

Qian, H, E Pang, J Chang, SL Toh, FK Ng, AL Tan and J Kwang: Monoclonal antibody binding to the major outer membrane protein of Campylobacter coli. Journal of Immunological Methods 339: 104-113 (2008).

Chang, J, E Pang, H He and J Kwang: Identification of novel attenuated Salmonella Enteritidis mutants. FEMS Immunology & Medical Microbiology 53: 26-34 (2008).

Qian, H, E Pang, Q Du, J Chang, J Dong, SL Toh, FK Ng, AL Tan and J Kwang: Production of a Monoclonal Antibody Specific for the Major Outer Membrane Protein of Campylobacter jejuni and Characterization of the Epitope. Applied and Environmental Microbiology 74: 833-839 (2008).

Velumani, S, Q Du, BJ Fenner, M Prabakaran, CW Lim, YN Lin and J Kwang: Development of an antigen-capture ELISA for detection of H7 subtype avian influenza from experimentally infected chickens. Journal of Virological Methods 147: 219-225 (2008).

Zhu, Y, A Lau, J Lau, Q Jia, AK Karuppannan and J Kwang: Enhanced replication of porcine circovirus type 2 (PCV2) in a homogeneous subpopulation of PK15 cell line. Virology 369: 423-430 (2007).

Liu, J, Y Zhu, I Chen, J Lau, F He, A Lau, Z Wang, AK Karuppannan and J Kwang: The ORF3 Protein of Porcine Circovirus Type 2 Interacts with Porcine Ubiquitin E3 Ligase Pirh2 and Facilitates p53 Expression in Viral Infection. Journal of Virology 81: 9560-9567 (2007).

Chen, SC, YC Chen, J Kwang, I Manopo, PC Wang, HC Chaung, LL Liaw and SH Chiu: Metschnikowia bicuspidata dominates in Taiwanese cold-weather yeast infections of Macrobrachium rosenbergii. Diseases of Aquatic Organisms 75: 191-199 (2007).

Fenner, BJ, W Goh and J Kwang: Dissection of Double-Stranded RNA Binding Protein B2 from Betanodavirus. Journal of Virology 81: 5449-5459 (2007).

He, Q, S Velumani, Q Du, CW Lim, FK Ng, R Donia and J Kwang: Detection of H5 Avian Influenza Viruses by Antigen-Capture Enzyme-Linked Immunosorbent Assay Using H5-Specific Monoclonal Antibody. Clinical and Vaccine Immunology 14: 617-623 (2007).

Fenner, BJ, Q Du, W Goh, R Thiagarajan, HK Chua and J Kwang: Detection of betanodavirus in juvenile barramundi, Lates calcarifer (Bloch), by antigen capture ELISA. Journal of Fish Diseases 29: 423-432 (2006).

Fenner, BJ, W Goh and J Kwang: Sequestration and Protection of Double-Stranded RNA by the Betanodavirus B2 Protein. Journal of Virology 80: 6822-6833 (2006).

Liu, J, I Chen, Q Du, H Chua and J Kwang: The ORF3 Protein of Porcine Circovirus Type 2 is Involved in Viral Pathogenesis in Vivo. Journal of Virology 80: 5065-5073 (2006).

Liu, J, I Chen, H Chua, Q Du and J Kwang: Inhibition of porcine circovirus type 2 replication in mice by RNA interference. Virology 347: 422-433 (2006).

He, F, BJ Fenner, AK Godwin and J Kwang: White Spot Syndrome Virus Open Reading Frame 222 Encodes a Viral E3 Ligase and Mediates Degradation of a Host Tumor Suppressor via Ubiquitination. Journal of Virology 80: 3884-3892 (2006).

Nwe, N, Q He, S Damrongwatanapokin, Q Du, I Manopo, Y Limlamthong, BJ Fenner, L Spencer and Jimmy Kwang: Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture. BMC Microbiology 6: 16 (2006).

Lu, L, Y Ho and J Kwang: Suppression of porcine arterivirus replication by baculovirus-delivered shRNA targeting nucleoprotein. Biochemical and Biophysical Research Communications 340: 1178-1183 (2006).

Fenner, BJ, R Thiagarajan, HK Chua and J Kwang: Betanodavirus B2 is an RNAi antagonist that facilitates intracellular viral RNA accumulation. Journal of Virology 80: 85-94 (2006)

Lu, Li, H Wang, I Manopo, L Yu and J Kwang: Baculovirus-mediated promoter assay and transcriptional analysis of white spot syndrome virus orf427 gene. Virology Journal 2: 71 (2005).

Wang, ZL, HK Chua, AARA Gusti, F He, B Fenner, I Manopo, H Wang and J Kwang: RING-H2 Protein WSSV249 from White Spot Syndrome Virus Sequesters a Shrimp Ubiquitin-Conjugating Enzyme, PvUbc, for Viral Pathogenesis. Journal of Virology 79: 8764-8772 (2005).

He, Q, QY Du, S Lau, I Manopo, L Lu, SW Chan, BJ Fenner and J Kwang: Characterization of Monoclonal Antibody Against SARS Coronavirus Nucleocapsid Antigen and Development of an Antigen Capture ELISA. Journal of Virological Methods 127: 46-53 (2005) .

Liu, J, I Chen and J Kwang: Characterization of a Previously Unidentified Viral Protein in PCV2 Infected Cells and Its Role in Virus-Induced Apoptosis. Journal of Virology. 79: 8262-8274 (2005).

He, Q, I Manopo, L Lu, BP Leung, HH Chng, AE Ling, LL Chee, SW Chan, EE Ooi, YL Sin, B Ang and J Kwang: Novel immunofluorescence assay using recombinant nucleocapsid-spike fusion protein as antigen to detect antibodies against severe acute respiratory syndrome coronavirus. Clinical and Diagnostic Laboratory Immunology 12: 321-328 (2005).

Manopo, I, L Lu, Q He, LL Chee, SW Chan and J Kwang: Evaluation of a safe and sensitive spike protein-based immunofluorescence assay for the detection of SARS-CoV infection. Journal of Immunological Methods 296: 37-44 (2005).

Ho, PS, J Kwang and YK Lee: Intragastric administration of Lactobacillus casei expressing transmissible gastroentritis coronavirus spike glycoprotein induced specific antibody production. Vaccine 11: 1335-1342 (2005).

Lu, L. and J. Kwang. 2004. Identification of a novel shrmip protein phosphatase and its association with latency related ORF427 of white spot syndrome virus. FEBS Letters. 577: 141-146.

Hossain, M.S., S. Khadijah and J. Kwang. 2004. Characterization of ORF89 - a latency related gene of white spot syndrom virus. Virology. 325:106-15.

Kwang, J. 2004. Microbes and livestocks. In: Microbial Biotechnology. ed. Y.K. Lee. World Scientific. pp319-390.

Lu, L., I. Manopo, B.P. Leung, H.H. Chng, A.E. Ling, L.L. Chee, E.E. Ooi, S.W. Chan and J. Kwang. 2004. Immunological characterization of the spike protein of Severe Acute Respiratory Syndrome coronavirus. Journal of Clinical Microbiology. 42: 1570-1576.

Guo, Y.X., S.W. Chan and J. Kwang. Membrane association of GGNNV protein A and characterization of its mitochondrial localization targeting signal. Journal of Virology. 78: 6498-6508.

Liu, J., T. Wei and J. Kwang. 2004. Membrane-association properties of avian encephalomyelitis virus protein 3A. Virology. 321: 297-306.

Seah, J.N. and J. Kwang. 2004. Localization of linear cytotoxic and pro-apoptotic epitopes in RTX toxin APxIII of Actinobacillus pleuropneumoniae. Vaccine. 22: 1494-1497.

He, Q., K.H. Chong, H.H. Chng, B. Leung, A.E. Ling, T. Wei, S.W. Chan, E.E. Ooi and J. Kwang. 2004. Development of a Western Blot Assay for Detection of Antibodies against Coronavirus Causing Severe Acute Respiratory Syndrome. Clinical and Diagnostic Laboratory Immunology. 11: 417-422.

Liu, J., T. Wei and J. Kwang. 2004. Avian encephalomyocarditis virus nonstructural protein 2C induces apoptosis by activating cytochrome c/caspase-9 pathway. Virology. 318: 169-182.

Khadijah, S., S. Y. Neo, M.S. Hossain, L.D. Miller, S. Mathavan and J. Kwang. 2003. Identification of White Spot Syndrome Virus latency-related genes in specific-pathogen-free shrimps by use of a microarray. Journal of Virology. 77: 10162-10167.

Yan Xiang Guo, Ting Wei, Klara Dallmann and Jimmy Kwang. 2003. Induction of caspase-dependent apoptosis by betanodaviruses GGNV and demonstration of protein alpha as an apoptotic inducer. Virology. 308: 74-82.

Yan Xiang Guo, Klara Dallmann and Jimmy Kwang. 2003. Identification of nucleolus localization signal of betanodavirus GGNNV protein alpha. Virology. 306: 225-235.

Y.T Wang, W. Liu, J.N Seah, C.S Lam, J.H Xiang, V. Korzh and J. Kwang. 2002. The white spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis. Diseases of Aquatic Organisms. 52: 249-259.

J.N. Seah, J. Frey and J. Kwang. 2002. The N-Terminal Domain of RTX Toxin ApxI of Actinobacillus pleuropneumoniae Elicits Protective Immunity in Mice. Infection and Immunity. 70: 6464-6467.

Engelsma MY, Hui Sing MO, Van Muiswinkel WB, Flick G, Kwang J, Savelkoul HFJ, Verburg-van Kemenade, BML. Neuroendocrine-immune interactions in fish: a role for interleukin-1. Veterinary Immunology and Immunopathology. 87: 467-479, 2002.

J. Liu, T. Wei, J. Kwang. Avian encephalomyelitis virus induces apoptosis via major structural protein VP3. Virology. 300: 39-49, 2002.

Liu J., Zhou J., Kwang J., Antigenic and Molecular Characterization of Recent Infectious Bursal Disease Virus Isolate in China. Virus Genes. 2:135-147, 2002.

Munday B L, J. Kwang, N. Moody. Nodavirus Infections of Teleost Fish: A Review. Journal of Fish Disease. 25: 127-142, 2002

Mathew J.A., Guo Y.X., Goh K.P., Chan J. Verburg-van Kemenade, B.M.L and Kwang J. (2001): Characterisation of a monoclonal antibody to carp IL-1B and the development of a sensitive capture ELISA. Fish & Shellfish Immunology (Accepted, Oct 2001)

Bin Huang, Sumanthi Subramaniam, Joachim Frey, Hilda Loh, Hai Meng Tan, Charlene J. Fernandez, Jimmy Kwang, Kim-Lee Chua. Vaccination of Ducks with Recombinant Outer Membrane Protein (OmpA) and a 41 kDa Partial protein (P45N') of Riemerella anatipestifer. Veterinary Microbiology. 84:219-230,2002.

Seah June Nam and Kwang J : Production of monoclonal antibody specific to Escherichia coli H33 flagellin by using predetermined polypeptides. Archives of Microbiology. 177:192-196, 2002

Liu W, YT Wang, DS Tian, ZC Yin and J Kwang : Detection of white spot syndrome virus (WSSV) of shrimp by means of monoclonal antibodies (MAbs) specific to an envelope protein (28Kda). Diseases of Aquatic Organisms. 49: 11-18, 2002.

J.R. Reddy, J. Kwang. K.F.Lechtenberg, N.C. Khan, B. Prasad Reddy, M.M. Chengappa. 2002. An Immunochromatographic serological assay for the diagnosis of Mycobacterium tuberculosis. Comparative Immunology, Microbiology & Infectious Diseases. 25:21-27.

Xiaodi Su, Sharon Low, Jimmy Kwang, Veronica H.T. Chew, Sam F.Y. Li. 2001. Piezoelectric quartz crystal based veterinary diagnosis for Salmonella enteritidis infection in chicken and egg. Sensors and Actuators B. 75: 29-35.

Seah June Nam and Kwang J (2001): Mapping of Escherichia coli H27 specific epitope from H-specific polypeptides. Clinical and Diagnostic Laboratory Immunology 8: 1126-1130, 2001.

Li Yu, Zhilian Wang, Yihai Jiang, Leo Chang, Jimmy Kwang (2001): Characterisation of newly emerging Newcastle disease virus isolates from the People's Republic of China and Taiwan. Journal of Clinical Microbiology 39, 3512-3519, 2001.

Liu W., Yang Y., Chung N., Kwang J. Induction of Humoral Immune Response and Protective Immunity in Chickens Against Salmonella enteritidis After a Single Dose of Killed Bacterium-Loaded Microspheres. Avian Disease 45:797-806, 2001

Puttur Mudumana Sudha, Sharon Low, Jimmy Kwang and Zhiyuan Gong (2001). Multiple tissue transformation in adult zebrafish by gene gun bombardment and muscular injection of naked DNA. Mar Biotechnol 3, 119-125, 2001

Wee G.J., Cao M.J., Liu W., Kwang J. (2001) Efficacy of Porcine Reproductive and Respiratory Syndrome Virus Vaccine and Porcine Interluekin 12 Vet. Therapeutics Vol 2 (2)p. 112-119 Spring 2001

Yap L.F., Low S., Liu W., Loh H., Teo T.P., Kwang J. (2001) Detection and screening of Salmonella enteritidis infected chickens using recombinant flagellin. Avian Diseases 45: 410-415.

Yu L., Jiang Y., Low S., Wang Z., Seah J.N., Liu W., Kwang J. (2001) Characterization of three infectious bronchitis virus isolates from China associated with proventriculus in vaccinated chickens. Avian Disease 45: 416-424.

Yu L., Liu W., Schnislein W.M., Tripathy D.N., Kwang J. (2001) Study of Protection by Recombinant Fowl Poxvirus Expressing C-Terminal Nucleocapsid Protein of Infectious Bronchitis Virus Against Challenge. Avian Diseases 45: 340-348.

Yu L., Wang Z., Jiang Y., Low S., Kwang J. (2001) Molecular epidemiology of Infectious Bronchitis Virus isolates from China and Southeast Asia. Avian Diseases 45: 201-209

Huang B., Tan C., Chang S.F., Munday B., J.A Mathew, Ngoh G.H., Kwang J. (2001) Detection of nodavirus in experimentally infected and commerical barramundi (Lates calcarifer) using recombinant coat protein-based ELISA and RT-PCR. J. Fish Dis 24:135-141.

Tan C., Huang B., Chang S.F., Ngoh G.H., Munday B., Chen S.C., Kwang J. (2001) Determination of the complete nucleotide sequence of the RNA1 and RNA2 of the Greasy grouper nervous necrosis virus. J. Gen. Virol. 82:647-653.

Tan C., Chang L., Shen S., Liu D.X., Kwang J. (2001) Comparison and determination of the 5'-leader sequences from seven different strains of porcine reproductive and respiratory syndrome virus (PRRSV). Virus Genes 22:209-217.

Seah J.N. & Kwang J. (2000) Identification of H-specific determinant in flagellin of four Escherichia coli strains. Arch. Microbiol. 174: 28-34.

Yin Z. & Kwang J (2000) Molecular cloning of carp transforming growth factor beta1 from activated leukocytes. Fish and Shellfish Immunol. 10: 379-388.

Yin Z. & Kwang J. (2000) Carp interleukin-1-beta in the role of an immuno-adjuvant. Fish and Shellfish Immunol. 10: 375-378.
Su X.D., Li S.F.Y., Liu W., Kwang J. (2000) Piezoelectric quartz crystal based screening test for porcine reproductive and respiratory syndrome virus infection in pigs. Analyst 125: 725-730.

Subramaniam S., Frey J., Huang B., Djordjevic S., Kwang J. (2000) Immunoblot assays using recombinant antigens for the detection of Mycoplasma hyopneumoniae. Vet. Microbiol. 75: 99-106.

Seah J.N. & Kwang J. (2000) Distribution of B-cell epitope on Infectious Bronchitis virus nucleocapsid. Vet. Microbiol. 75: 11-16.

Yin Z. & Kwang J. (2000) Molecular cloning of a carp Jak3 from activated leukocytes. Vet. Immunol. Immunopathol. 74: 225-236.

Kwang J. (2000). Fishing for vaccines. Nature Biotechnology 18: 1145-1146.