Srinivasan RAMACHANDRAN, Ph.D, Senior Principal Investigator

Srinivasan Ramachandran obtained his PhD in Biochemistry with Plant Molecular Biology as specialization from University of Idaho under the guidance of Prof David Oliver. In 1993 he joined Prof Nam Hai Chua’s group at the Institute of Molecular and Cell Biology and Institute of Molecular Agrobiology, Singapore. Since 2001 he is heading the group of Rice Functional Genomics at Institute of Molecular Biology and since August 2002 at the Temasek Life Sciences Laboratory.

You may wish to contact Dr Srinivasan RAMACHANDRAN at: Tel: (65) 6872 7000,  6872 7480 (DID) or 6872 7489 (lab) Email: sri@tll.org.sg

 

For information on PhD studies at TLL, click HERE

Research Interests

  • Establish gene tagging system using maize Ac/Ds transposon in rice
  • Study the Ac/Ds system behavior in rice
  • Identify and characterize agronomic trait mutants with altered morphological and yield characteristics
  • Characterize abiotic stress (drought, salinity and cold) mutants and dissect the stress mediated signal transduction in rice
  • Study the rice genome with respect to various gene families using bioinformatics tools.

Research Projects

Several approaches can be used to study the functions of genes in an organism. Examples of successful approaches used in different organisms include gene replacement, anti-sense and co-suppression technologies, insertional mutagenesis, and microarrays. In plants, random insertional mutagenesis by either T-DNA or transposable elements has to date been the most successful strategy to identify gene functions on a genome-wide scale. Our group focuses on the functional genomics of rice by the method of transposon insertional mutagenesis.

Rice, which is a plant of considerable agronomical importance, has also become a model system for all of the cereals for several reasons, such as its relatively small genome (430Mb) which will be completely sequenced in the next few years, the availability of dense genetic and molecular maps, and ease of transformation etc. Our objective is to generate a large population of tagged genes in rice which can be analyzed for reporter gene expression and mutant phenotypes, and establish a database of flanking sequence tags (FSTs). Prof. Venkatesan Sundaresan’s group has previously used a transposable element gene trapping system in the model plant Arabidopsis thaliana to generate a large collection of gene trap insertions, and establish a database of insertions with flanking sequence. For rice, we have successfully developed modified versions of the Ac/Ds transposon system that have been proven effective in Arabidopsis. These systems are being used to generate a similar collection of transposon insertions in rice, which will form the basis for large-scale functional genomics in this important crop plant.